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sybrgreen fluorescent detection dye system  (PrimerDesign Inc)

 
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    PrimerDesign Inc sybrgreen fluorescent detection dye system
    Sybrgreen Fluorescent Detection Dye System, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen fluorescent detection dye system/product/PrimerDesign Inc
    Average 90 stars, based on 1 article reviews
    sybrgreen fluorescent detection dye system - by Bioz Stars, 2026-03
    90/100 stars

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    Relative standard curves. The standard curves were generated by the Mx3000P software by plotting cycles at threshold fluorescence (Ct) against the logarithmic values of standard RNA amounts. Quantities of standard RNA were expressed as dilution factors of the RNA preparation (1, 0.5, 0.25, 0.125, 0.0125, 0.00125). Correlation coefficients (square values – r 2 ) and amplification efficiencies (E) are shown. SYBRGreen I fluorescences were corrected with passive reference dye (ROX) fluorescences („dRn“).

    Journal: BMC Molecular Biology

    Article Title: Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

    doi: 10.1186/1471-2199-8-113

    Figure Lengend Snippet: Relative standard curves. The standard curves were generated by the Mx3000P software by plotting cycles at threshold fluorescence (Ct) against the logarithmic values of standard RNA amounts. Quantities of standard RNA were expressed as dilution factors of the RNA preparation (1, 0.5, 0.25, 0.125, 0.0125, 0.00125). Correlation coefficients (square values – r 2 ) and amplification efficiencies (E) are shown. SYBRGreen I fluorescences were corrected with passive reference dye (ROX) fluorescences („dRn“).

    Article Snippet: PCR reactions were carried out in duplicates using SYBRGreen I as a fluorescent detection dye and they were performed in the real-time PCR system Mx 3000P (Stratagene, La Jolla, CA).

    Techniques: Generated, Software, Fluorescence, Amplification